Molecular Formula | mSiO2·nH2O |
Appearance | White powder |
Storage Condition | Room Temprature |
Physical and Chemical Properties | The chemical properties are white granular coarse-porous silica gel. Insoluble in water and other organic solvents. |
Use | It is used for the purification of active ingredients of Chinese herbal medicine, the preparation of high-purity substances, the refining of petrochemical products, the purification of nucleic acids, and the preparation of urokinase by adsorption. |
Use of column chromatography silica gel
column chromatography silica gel is separated according to the different adsorption forces of substances on silica gel. under normal circumstances, substances with larger polarity are easy to be adsorbed by silica gel, while substances with weaker polarity are not easy to be adsorbed by silica gel. the whole chromatography process is the process of adsorption, desorption, re-adsorption and re-desorption. Column layer chromatography silica gel is a colloidal system with solid characteristics, which is composed of colloidal particles forming agglutination structure. Colloidal particles are condensed polymers of hydrated silica gel (polysilicic acid), which are amorphous substances. The gap of the aggregate of colloidal particles forms the micropore structure inside the silica gel particles by reagent column chromatography. Therefore, column layer chromatography silica gel is a kind of high-quality adsorption material with rich microporous structure, high specific surface area, high purity and high activity.
the microstructure of column layer chromatography silica gel is not different from that of general silica gel. SiO2, which forms the colloidal skeleton, is combined with silicon oxygen tetrahedron, and the force field between atoms is balanced. As mentioned earlier, silica gel has a high specific surface area. The surface structure of the internal pores of silica gel particles is different from the internal structure of the skeleton formed. The silicon atoms on the surface and the structural water contained in the colloid form a silanol group. The imbalance of this structure makes the surface of silica gel produce a free force field, that is, it has the ability to adsorb water molecules or other polar molecules. The adsorbed substances are different due to the polarity of the molecules, the adsorption force on the surface of colloidal particles varies to varying degrees. For this reason, silica gel is selective in adsorption of mixtures of different substances. When the material component with strong molecular polarity passes through the surface of silica gel, the adsorption force generated by silica gel is also strong, and the retention time of the material component on the surface of silica gel is longer; on the contrary, the retention time of the component with weak molecular polarity is shorter. Therefore, the mixture of different substances is separated due to the difference in retention time during the passage of silica gel. For substances with strong molecular polarity, silica gel has a strong adsorption capacity, such as water molecules. In this case, the adsorbed substance molecules can only overcome the barrier of the gravitational field generated by the silica gel surface and break away from the silica gel surface when sufficient energy (such as thermal energy) is obtained. In this way, under normal conditions, when a mixture containing a strongly polar substance component passes through a column layer of silica gel, the strongly polar substance component is retained inside the pores of the silica gel, thus exhibiting the ability of the silica gel to dehydrate or purify the substance.
Production method
coarse-hole silica gel is crushed to 40~200 mesh by impregnation method, screened, impregnated with hydrochloric acid for 20h, washed with water, dried until the water content is less than or equal to 5%, and column chromatography silica gel is prepared.